Development of a Rapid RT-PCR Test for the Detection of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid and Apple Scar Skin Viroid in Fruit Trees from Tunisia
نویسندگان
چکیده
منابع مشابه
A Viroid Resembling Hop Stunt Viroid in Infected Apple Trees with Apple Scar Skin Disease in the Northeast of Iran
Abstract Background and Aims: Apple scar skin disease (ASSD) is one of the destructive diseases of pome fruits which is caused by ASSVd. Case presentation: We report molecular detection of ASSVd in symptomatic apples cv. Red Delicious with simultaneous presence of a new variant of HSVd from Khorasan Razavi province. Conclusion: This is the first report on the association of HSVd with...
متن کاملogenicity, viroid evolution, Citrus exocortis viroid, Potato spindle tuber viroid, Peach latent mosaic viroid, Chrysan- themum chlorotic mottle viroid
Viroids are autonomously replicating, small singlestranded circular RNA molecules that do not code for proteins and may cause disease in infected, susceptible plants. Viroids have the ability to induce both RNA-mediated transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS) in infected plants. Viroid PTGS has also been demonstrated in a wheat germ extract system. A p...
متن کاملa viroid resembling hop stunt viroid in infected apple trees with apple scar skin disease in the northeast of iran
apple scar skin disease (assd) is one of the destructive diseases of pome fruits which is caused by assvd. we report molecular detection of assvd in symptomatic apples cv. red delicious with simultaneous presence of a new variant of hsvd from khorasan razavi province. this is the first report on the association of hsvd with assvd in apple trees showing merely symptoms of apple dapple on the fruit.
متن کاملGenomic structure of new Tunisian peach latent mosaic viroid variants.
Peach latent mosaic viroid (PLMVd) is a single-stranded circular RNA that do not code for proteins and ranges in size from 335 to 351 nucleotides. It mainly infects peach. In this study, the sequence of 20 complete cDNA clones derived from seven PLMVd isolates detected in five Tunisian peach cultivars was analysed in 3 steps: primary structure, phylogeny and secondary structure. The analysis of...
متن کاملNucleotide sequence and secondary structure of apple scar skin viroid.
The complete nucleotide sequence of apple scar skin viroid(ASSV) has been established, and a probable secondary structure is proposed. A single-stranded circular ASSV RNA consists of 330 nucleotides and can assume the rodlike conformation with extensive base-pairing characteristic of all the known viroids. ASSV shows low sequence homologies with other viroids and lacks the central conserved reg...
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ژورنال
عنوان ژورنال: Journal of Phytopathology
سال: 2006
ISSN: 0931-1785,1439-0434
DOI: 10.1111/j.1439-0434.2006.01087.x